ncRNA name | GCAP1 shRNA |
ncRNA Category | shRNA |
Disease name | retinitis pigmentosa (RP) |
Species | Mus musculus |
Tissues/Cell_line | retinas |
Methods | shRNA knockdown, immunoblot, qRT-PCR, Western blot, fluorescence imaging, ERG, immunohistochemistry |
Expression pattern | associated |
Functional description | We established an in vitro shRNA screening assay based on EGFP-tagged bovine GCAP1, and identified a shRNA that effectively silenced the bovine GCAP1 transgene with ∼80% efficiency. The gene silencing significantly improved photoreceptor survival, delayed disease onset, and increased visual function. |
PubMed ID | 22042849 |
Year | 2011 |
Title | Long-term RNA interference gene therapy in a dominant retinitis pigmentosa mouse model. |
Drug-related ncRNA | NA |
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